An Evolved Mxe GyrA Intein for Enhanced Production of Fusion Proteins
نویسندگان
چکیده
منابع مشابه
An Evolved Mxe GyrA Intein for Enhanced Production of Fusion Proteins
Expressing antibodies as fusions to the non-self-cleaving Mxe GyrA intein enables site-specific, carboxy-terminal chemical modification of the antibodies by expressed protein ligation (EPL). Bacterial antibody-intein fusion protein expression platforms typically yield insoluble inclusion bodies that require refolding to obtain active antibody-intein fusion proteins. Previously, we demonstrated ...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2014
ISSN: 1554-8929,1554-8937
DOI: 10.1021/cb500689g